GTF2A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16760

Novus Biologicals, part of Bio-Techne
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NBP2-16760
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GTF2A1 Antibodies »

Product Summary for GTF2A1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human GTF2A1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus tropicalis (81%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GTF2A1 Antibody

Western Blot: GTF2A1 Antibody [NBP2-16760]

Western Blot: GTF2A1 Antibody [NBP2-16760]

Western Blot: GTF2A1 Antibody [NBP2-16760] - Sample (30 ug of whole cell lysate) A: 293T B: HeLa 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody [NBP2-16760]

Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody [NBP2-16760]

Immunocytochemistry/Immunofluorescence: GTF2A1 Antibody [NBP2-16760] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GTF2A1 Antibody [NBP2-16760]

Immunohistochemistry-Paraffin: GTF2A1 Antibody [NBP2-16760]

Immunohistochemistry-Paraffin: GTF2A1 Antibody [NBP2-16760] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.

Applications for GTF2A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GTF2A1

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM]

Alternate Names

General transcription factor IIA subunit 1, general transcription factor IIA, 1, 19/37kDa, glucose regulated protein, 58kD pseudogene, MGC129969, MGC129970, TF2A1, TFIIA, TFIIA alpha, p55, TFIIA-42, TFIIAL, transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit

Gene Symbol

GTF2A1

UniProt

P52655 (Human)

Additional GTF2A1 Products

Product Documents for GTF2A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GTF2A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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