GTF2A2 Antibody (2B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00002958-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GTF2A2 Antibodies »

Product Specifications

Immunogen

GTF2A2 (AAH00287, 1 a.a. ~ 109 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINAALAQRVRNRVNFRGSLNTYRFCDNVWTFVLNDVEFREVTELIKVDKVKIVACDGKNTGSNTTE

Specificity

GTF2A2 - general transcription factor IIA, 2, 12kDa (2B9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GTF2A2 Antibody (2B9)

Western Blot: GTF2A2 Antibody (2B9) [H00002958-M01]

Western Blot: GTF2A2 Antibody (2B9) [H00002958-M01]

Western Blot: GTF2A2 Antibody (2B9) [H00002958-M01] - Analysis of GTF2A2 expression in transfected 293T cell line by GTF2A2 monoclonal antibody (M01), clone 2B9.Lane 1: GTF2A2 transfected lysate(12.5 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: GTF2A2 Antibody (2B9) [H00002958-M01] - Detection limit for recombinant GST tagged GTF2A2 is approximately 0.3ng/ml as a capture antibody.

Applications for GTF2A2 Antibody (2B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GTF2A2

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM]

Alternate Names

General transcription factor IIA subunit 2, general transcription factor IIA, 2 (12kD subunit), general transcription factor IIA, 2, 12kDa, HsT18745, TF2A2, TFIIA, TFIIA p12 subunit, TFIIA-12, TFIIA-gamma, TFIIAS, Transcription initiation factor IIA gamma chain, transcription initiation factor IIA subunit 2

Entrez Gene IDs

2958 (Human)

Gene Symbol

GTF2A2

UniProt

AAH00287 (Human)

Additional GTF2A2 Products

Product Documents for GTF2A2 Antibody (2B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GTF2A2 Antibody (2B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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