GTF3A Antibody (4B4)

Novus Biologicals, part of Bio-Techne | Catalog # H00002971-M10

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4B4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GTF3A Antibodies »

Product Specifications

Immunogen

GTF3A (NP_002088, 185 a.a. ~ 274 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NQQKQYICSFEDCKKTFKKHQQLKIHQCQNTNEPLFKCTQEGCGKHFASPSKLKRHAKAHEGYVCQKGCSFVAKTWTELLKHVRETHKEE

Specificity

GTF3A (4B4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GTF3A Antibody (4B4)

ELISA: GTF3A Antibody (4B4) [H00002971-M10]

ELISA: GTF3A Antibody (4B4) [H00002971-M10]

ELISA: GTF3A Antibody (4B4) [H00002971-M10] - Detection limit for recombinant GST tagged GTF3A is approximately 30ng/ml as a capture antibody.

Applications for GTF3A Antibody (4B4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GTF3A

The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein.

Alternate Names

general transcription factor IIIA, TFIIIAAP2, transcription factor IIIA

Entrez Gene IDs

2971 (Human)

Gene Symbol

GTF3A

OMIM

600860 (Human)

UniProt

NP_002088 (Human)

Additional GTF3A Products

Product Documents for GTF3A Antibody (4B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GTF3A Antibody (4B4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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