Guanylate kinase Antibody (OTI4A8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00594

Novus Biologicals, part of Bio-Techne
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NBP2-00594
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Conjugate
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Alexa Fluor 350
NBP2-70869AF350
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Alexa Fluor 405
NBP2-70869AF405
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Alexa Fluor 488
NBP2-70869AF488
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Alexa Fluor 532
NBP2-70869AF532
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Alexa Fluor 594
NBP2-70869AF594
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Alexa Fluor 647
NBP2-70869AF647
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Alexa Fluor 700
NBP2-70869AF700
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Alexa Fluor 750
NBP2-70869AF750
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Allophycocyanin
NBP2-70869APC
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Allophycocyanin/Cy7
NBP2-70869APCCY7
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Biotin
NBP2-70869B
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CoraFluor 1
NBP2-70869CL1
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DyLight 350
NBP2-70869UV
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DyLight 405
NBP2-70869V
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DyLight 488
NBP2-70869G
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DyLight 550
NBP2-70869R
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DyLight 594
NBP2-70869DL594
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DyLight 650
NBP2-70869C
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DyLight 680
NBP2-70869FR
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DyLight 755
NBP2-70869IR
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FITC
NBP2-70869F
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HRP
NBP2-70869H
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Janelia Fluor 525
NBP2-70869JF525
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Janelia Fluor 549
NBP2-70869JF549
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Janelia Fluor 585
NBP2-70869JF585
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Janelia Fluor 635
NBP2-70869JF635
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Janelia Fluor 646
NBP2-70869JF646
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Janelia Fluor 669
NBP2-70869JF669
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mFluor Violet 450 SE
NBP2-70869MFV450
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mFluor Violet 500 SE
NBP2-70869MFV500
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mFluor Violet 610 SE
NBP2-70869MFV610
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PE
NBP2-70869PE
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PE/Atto594
NBP2-70869PEATT594
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PE/Cy5.5
NBP2-70869PECY55
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PE/Cy7
NBP2-70869PECY7
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PerCP
NBP2-70869PCP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4A8

Concentration

0.47 mg/ml

View all Guanylate Kinase Antibodies »

Product Summary for Guanylate kinase Antibody (OTI4A8)

Immunogen

Full length human recombinant protein of human GUK1 (NP_000849) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Guanylate kinase Antibody (OTI4A8)

Western Blot: Guanylate kinase Antibody (OTI4A8) [NBP2-00594]

Western Blot: Guanylate kinase Antibody (OTI4A8) [NBP2-00594]

Western Blot: Guanylate kinase Antibody (4A8) [NBP2-00594] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Guanylate kinase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Guanylate kinase.
Immunohistochemistry-Paraffin: Guanylate kinase Antibody (4A8) [NBP2-00594] Staining of paraffin-embedded Human prostate tissue using anti-Guanylate kinase mouse monoclonal antibody.
Flow Cytometry: Guanylate kinase Antibody (OTI4A8) [NBP2-00594]

Flow Cytometry: Guanylate kinase Antibody (OTI4A8) [NBP2-00594]

Flow Cytometry: Guanylate kinase Antibody (4A8) [NBP2-00594] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Guanylate kinase antibody, and then analyzed by flow cytometry.
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Applications for Guanylate kinase Antibody (OTI4A8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.47 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Guanylate Kinase

Essential for recycling GMP and indirectly, cGMP

Alternate Names

ATP:GMP Phosphotransferase, Deoxyguanylate Kinase, GMK, GMP Kinase, Guanosine monophosphate Kinase, GUK1

Entrez Gene IDs

2987 (Human)

Gene Symbol

GUK1

Additional Guanylate Kinase Products

Product Documents for Guanylate kinase Antibody (OTI4A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Guanylate kinase Antibody (OTI4A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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