Guanylyl Cyclase beta 1 Antibody (5O8D9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16248

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16248-100ul
NBP3-16248-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5O8D9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Guanylyl Cyclase beta 1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Guanylyl Cyclase beta 1 (Q02153). MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Guanylyl Cyclase beta 1 Antibody (5O8D9)

Western Blot: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248]

Western Blot: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248]

Western Blot: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248] - Analysis of extracts of various cell lines, using Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit mAb (NBP3-16248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248]

Immunocytochemistry/ Immunofluorescence: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248]

Immunocytochemistry/Immunofluorescence: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248] - Immunofluorescence analysis of NIH-3T3 cells using Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit mAb (NBP3-16248) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248]

Immunohistochemistry-Paraffin: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248]

Immunohistochemistry-Paraffin: Guanylyl Cyclase beta 1 Antibody (5O8D9) [NBP3-16248] - Human brain using Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit mAb (NBP3-16248) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Guanylyl Cyclase beta 1 Antibody (5O8D9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 -1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Guanylyl Cyclase beta 1

Guanylate Cyclase is an heterodimer of an alpha and a beta chain. Its enzymatic activity transforms GTP to GMP releasing a diphosphate. It binds 1 or 2 hemes per heterodimer. It is activated by nitric oxide in the presence of magnesium or manganese ions. There are two types of guanylate cyclase: soluble form and membrane-associated receptor form.

Alternate Names

EC 4.6.1.2, GC-SB3, GCS-beta-1, GCS-beta-3, guanylate cyclase 1, soluble, beta 3, guanylate cyclase soluble subunit beta-1, Guanylate cyclase soluble subunit beta-3, GUC1B3GUCB3, GUCSB3GC-S-beta-1, GUCY1B1, Soluble guanylate cyclase small subunit

Gene Symbol

GUCY1B1

Additional Guanylyl Cyclase beta 1 Products

Product Documents for Guanylyl Cyclase beta 1 Antibody (5O8D9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Guanylyl Cyclase beta 1 Antibody (5O8D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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