Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GW182 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1225 and 1275 of human glycine-tryptophan protein of 182 kDa (GW182) using the numbering given in entry NP_055309.2 (GeneID 27327).

Marker

P/GW Body Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GW182 Antibody

Immunoprecipitation: GW182 Antibody [NBP1-28702]

Immunoprecipitation: GW182 Antibody [NBP1-28702]

Immunoprecipitation: GW182 Antibody [NBP1-28702] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28702 used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28751, which recognizes an upstream epitope. For blotting immunoprecipitated GW182, NBP1-28751 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: GW182 Antibody [NBP1-28702]

Immunoprecipitation: GW182 Antibody [NBP1-28702]

Immunoprecipitation: GW182 Antibody [NBP1-28702] - Immunoprecipitation on mouse brain samples - (dissected hippocampus). The recommended dilution is 1:100.

Applications for GW182 Antibody

Application
Recommended Usage

Immunoprecipitation

2 -5 ug/mg lysate
Application Notes
Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GW182

GW182 (glycine-tryptophan protein of 182 kDa) is a member of the trinucleotide repeat containing 6 protein family. It functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. GW182 has been found to associate with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibition of GW182 expression delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.

Alternate Names

CAG repeat protein 26, CAGH26FLJ22043, DKFZp666E117, EMSY interactor protein, Glycine-tryptophan protein of 182 kDa, GW182 autoantigen, GW182GW1, KIAA1460EDIE, MGC75384, Protein GW1, TNRC6, trinucleotide repeat containing 6, trinucleotide repeat containing 6A, trinucleotide repeat-containing gene 6A protein

Entrez Gene IDs

27327 (Human)

Gene Symbol

TNRC6A

UniProt

Q8NDV7 (Human)

Additional GW182 Products

Product Documents for GW182 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GW182 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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