GW182 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03014
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for GW182 Antibody - BSA Free
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human GW182 (NP_055309.2). QDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAIS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GW182 Antibody - BSA Free
![Western Blot: GW182 AntibodyBSA Free [NBP3-03014]](https://resources.bio-techne.com/images/products/GW182-Antibody-Western-Blot-NBP3-03014-img0002.jpg "Western Blot: GW182 AntibodyBSA Free [NBP3-03014]")
Western Blot: GW182 AntibodyBSA Free [NBP3-03014]
Western Blot: GW182 Antibody [NBP3-03014] - Analysis of extracts of various cell lines, using GW182 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit![Immunocytochemistry/ Immunofluorescence: GW182 Antibody - BSA Free [NBP3-03014]](https://resources.bio-techne.com/images/products/GW182-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03014-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GW182 Antibody - BSA Free [NBP3-03014]")
Immunocytochemistry/ Immunofluorescence: GW182 Antibody - BSA Free [NBP3-03014]
Immunocytochemistry/Immunofluorescence: GW182 Antibody [NBP3-03014] - Analysis of U2OS cells using GW182 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Applications for GW182 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GW182
Alternate Names
CAG repeat protein 26, CAGH26FLJ22043, DKFZp666E117, EMSY interactor protein, Glycine-tryptophan protein of 182 kDa, GW182 autoantigen, GW182GW1, KIAA1460EDIE, MGC75384, Protein GW1, TNRC6, trinucleotide repeat containing 6, trinucleotide repeat containing 6A, trinucleotide repeat-containing gene 6A protein
Gene Symbol
TNRC6A
Additional GW182 Products
Product Documents for GW182 Antibody - BSA Free
Product Specific Notices for GW182 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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