H-2Dd Antibody (34-5-8S) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28186

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 34-5-8S

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all H-2Dd Antibodies »

Product Specifications

Immunogen

BDF1 mouse splenocytes

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for H-2Dd Antibody (34-5-8S) [FITC]

Flow Cytometry: H-2Dd Antibody (34-5-8S) [FITC] [NBP1-28186] - BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Dd-FITC.
Flow Cytometry: H-2Dd Antibody (34-5-8S) [FITC] [NBP1-28186] - Analysis of DBA/2 splenocytes.

Applications for H-2Dd Antibody (34-5-8S) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: H-2Dd

MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpaha1 and alpha2 chains when complexed with beta2-microglobulin. (2) The antibody does not react with H-2Dd alpha chains synthesized in vitro. (3) Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. (4) Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. (2) MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells. (5-9)

Alternate Names

H2Dd, MHC class I

Additional H-2Dd Products

Product Documents for H-2Dd Antibody (34-5-8S) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for H-2Dd Antibody (34-5-8S) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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