H1F0 Antibody (5E3Q8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16378
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5E3Q8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for H1F0 Antibody (5E3Q8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human H1F0 (P07305). MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for H1F0 Antibody (5E3Q8)
![Western Blot: H1F0 Antibody (5E3Q8) [NBP3-16378]](https://resources.bio-techne.com/images/products/H1F0-Antibody-5E3Q8-Western-Blot-NBP3-16378-img0002.jpg "Western Blot: H1F0 Antibody (5E3Q8) [NBP3-16378]")
Western Blot: H1F0 Antibody (5E3Q8) [NBP3-16378]
Western Blot: H1F0 Antibody (5E3Q8) [NBP3-16378] - Western blot analysis of extracts of various cell lines, using H1F0 Rabbit mAb (NBP3-16378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry-Paraffin: H1F0 Antibody (5E3Q8) [NBP3-16378]](https://resources.bio-techne.com/images/products/H1F0-Antibody-5E3Q8-Immunohistochemistry-Paraffin-NBP3-16378-img0001.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (5E3Q8) [NBP3-16378]")
Immunohistochemistry-Paraffin: H1F0 Antibody (5E3Q8) [NBP3-16378]
Immunohistochemistry-Paraffin: H1F0 Antibody (5E3Q8) [NBP3-16378] - Immunohistochemistry of paraffin-embedded human thyroid cancer using H1F0 Rabbit mAb (NBP3-16378) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for H1F0 Antibody (5E3Q8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: H1F0
Alternate Names
H1 histone family, member 0, H1.0, H1(0), H1-0, H10, H1FVMGC5241, Histone H1', Histone H1(0), histone H1.0
Gene Symbol
H1-0
Additional H1F0 Products
Product Documents for H1F0 Antibody (5E3Q8)
Product Specific Notices for H1F0 Antibody (5E3Q8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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