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H1F0 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83308

Novus Biologicals, part of Bio-Techne
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NBP1-83308

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for H1F0 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MTENSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for H1F0 Antibody

Western Blot: H1F0 Antibody [NBP1-83308]

Western Blot: H1F0 Antibody [NBP1-83308]

Western Blot: H1F0 Antibody [NBP1-83308] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line U-2 OS
Immunocytochemistry/ Immunofluorescence: H1F0 Antibody [NBP1-83308]

Immunocytochemistry/ Immunofluorescence: H1F0 Antibody [NBP1-83308]

Immunocytochemistry/Immunofluorescence: H1F0 Antibody [NBP1-83308] - Staining of human cell line A-431 shows localization to nucleus & actin filaments.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308]

Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining in human prostate and lymph node tissues using anti-H1F0 antibody. Corresponding H1F0 RNA-seq data are presented for the same tissues.

Applications for H1F0 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: H1F0

Plasmid: pCMV-H1F0 Full length; Host Cell: 293T

Alternate Names

H1 histone family, member 0, H1.0, H1(0), H1-0, H10, H1FVMGC5241, Histone H1', Histone H1(0), histone H1.0

Gene Symbol

H1-0

Additional H1F0 Products

Product Documents for H1F0 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for H1F0 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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