H1FOO Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86265

Novus Biologicals, part of Bio-Techne
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NBP1-86265
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all H1FOO Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YRKTKAESKSSKPTASKVKNGAASPTKKKVVAKAKAPKAGQGPNTKAAAPAKGSGSKVVPAHLSRKTEAPKGPRKAGLPIKASSSKVSSQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for H1FOO Antibody

Western Blot: H1FOO Antibody [NBP1-86265]

Western Blot: H1FOO Antibody [NBP1-86265]

Western Blot: H1FOO Antibody [NBP1-86265] - Analysis in control (vector only transfected HEK293T lysate) and H1FOO over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: H1FOO Antibody [NBP1-86265]

Immunohistochemistry-Paraffin: H1FOO Antibody [NBP1-86265]

Immunohistochemistry-Paraffin: H1FOO Antibody [NBP1-86265] - Staining of human Testis shows moderate nuclear positivity in spermatids.
Immunohistochemistry-Paraffin: H1FOO Antibody [NBP1-86265]

Immunohistochemistry-Paraffin: H1FOO Antibody [NBP1-86265]

Immunohistochemistry-Paraffin: H1FOO Antibody [NBP1-86265] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
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Applications for H1FOO Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: H1FOO

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The H1FOO gene encodes a H1 Histone Family, member O, oocyte-specific protein. One isoform is 346 amino acids long at 35 kDA while isoform 2 is 207 amino acids long at 21 kDA. The replacement of H1c with H1FOO may be critical to nuclear remodeling as greater mobility of H1FOO increases stability of the embryonic chromatin structure. H1FOO participates in histone modification and cell cycle chromosome condensation in prometaphase. H1FOO has been studied in regards to premature ovarian failure.

Alternate Names

H1 histone family, member O, oocyte-specific, H1OO, histone H1oo, MGC50807, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1

Gene Symbol

H1FOO

Additional H1FOO Products

Product Documents for H1FOO Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for H1FOO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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