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H2A Antibody (4C10)

Novus Biologicals, part of Bio-Techne | Catalog # H00008337-M01

Novus Biologicals, part of Bio-Techne
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H00008337-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HIST2H2AA (AAH01629, 1 a.a. ~ 130 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Specificity

HIST2H2AA - histone 2, H2aa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for H2A Antibody (4C10)

Immunocytochemistry/ Immunofluorescence: H2A Antibody (4C10) [H00008337-M01]

Immunocytochemistry/ Immunofluorescence: H2A Antibody (4C10) [H00008337-M01]

Immunocytochemistry/Immunofluorescence: H2A Antibody (4C10) [H00008337-M01] - Analysis of monoclonal antibody to HIST2H2AA3 on HeLa cell . Antibody concentration 10 ug/ml.

Applications for H2A Antibody (4C10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: H2A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Alternate Names

H2A histone family, member O, H2A.2, H2A/O, H2A/q, H2a-615, H2AFOHistone H2A/o, HIST2H2AAH2A, histone 2, H2aa, histone 2, H2aa3, histone cluster 2, H2aa3, histone H2A type 2-A, Histone H2A.2

Entrez Gene IDs

8337 (Human)

Gene Symbol

HIST2H2AA3

OMIM

142720 (Human)

Additional H2A Products

Product Documents for H2A Antibody (4C10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for H2A Antibody (4C10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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