HA Tag Antibody (12CA5[2])
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50701
Key Product Details
Species Reactivity
Epitope Tag
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 12CA5[2]
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This HA Tag Antibody (12CA5[2]) was developed against the synthetic peptide (YPYDVPDYA) derived from the human influenza hemaglutinin protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab1424)
Applications for HA Tag Antibody (12CA5[2])
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 - 20 ug/mL
Immunoprecipitation
10 - 20 ug/mL
Western Blot
0.5 - 4 ug/mL
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2), 50% glycerol, 1% BSA
Preservative
0.02% Thimerosal
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HA Tag
References
1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076
2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778
3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093
4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73
5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107
Alternate Names
HA Epitope Tag
Additional HA Tag Products
Product Documents for HA Tag Antibody (12CA5[2])
Product Specific Notices for HA Tag Antibody (12CA5[2])
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.