HA Tag Antibody (C.15200190)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59172
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # C.15200190
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HA Tag Antibody (C.15200190)
Immunogen
This HA Tag Antibody (C.15200190) was raised in mouse against the HA tag (amino acid sequence YPYDVPDYA) using a KLHconjugated synthetic peptide.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Description
The HA antibody recognizes the influenza Hemagglutinin epitope which is extensively used as a protein tag in expression vectors. This antibody is extremely specific and allows unambiguous identification and quantitative analysis of HA tagged proteins.
Scientific Data Images for HA Tag Antibody (C.15200190)
![Western Blot: HA Tag Antibody (C.15200190) [NBP2-59172]](https://resources.bio-techne.com/images/products/HA-Tag-Antibody-C-15200190-Western-Blot-NBP2-59172-img0001.jpg "Western Blot: HA Tag Antibody (C.15200190) [NBP2-59172]")
Western Blot: HA Tag Antibody (C.15200190) [NBP2-59172]
Western Blot: HA Tag Antibody (C.15200190) [NBP2-59172] - Whole cell extracts from HEK293 cells transfected with an HA-tagged expression vector using the antibody directed against HA at 1:1,000. Figure shows the results for untransfected cells, used as a negative control (lane 1) and for cells transfected with a HA-tagged protein (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.Applications for HA Tag Antibody (C.15200190)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:150-1:200
Immunoprecipitation
1:150 - 1:200
Western Blot
1:1000 - 1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HA Tag
References
1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076
2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778
3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093
4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73
5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107
Alternate Names
HA Epitope Tag
Additional HA Tag Products
Product Documents for HA Tag Antibody (C.15200190)
Product Specific Notices for HA Tag Antibody (C.15200190)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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