HA Tag Antibody (RM305)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61477

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Epitope Tag

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM305

Concentration

1 mg/ml

View all HA Tag Antibodies »

Product Specifications

Immunogen

This HA Tag Antibody (RM305) was developed against a peptide corresponding to HA Tag (Human influenza hemagglutinin amino acids 98-106).

Specificity

This antibody reacts to recombinant proteins containing a HA Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HA Tag Antibody (RM305)

Western Blot: HA Tag Antibody (RM305) [NBP2-61477] -

Western Blot: HA Tag Antibody (RM305) [NBP2-61477] - 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61477 at 0.1 ug/ml

Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) [NBP2-61477] -

Immunohistochemistry-Paraffin: HA Tag Antibody (RM305) [NBP2-61477] - Staining of LNCaP cells overexpressing HA-tag Bag1 protein, using NBP2-61477 at 0.01 ug/ml

Applications for HA Tag Antibody (RM305)

Application
Recommended Usage

Flow Cytometry

0.1 - 1 ug/mL

Immunocytochemistry/ Immunofluorescence

0.1 - 1 ug/mL

Immunohistochemistry

0.01 - 0.5 ug/ml

Immunoprecipitation

0.5 ug/ml - 2 ug/ml

Western Blot

0.1 ug/ml - 1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HA Tag

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Alternate Names

HA Epitope Tag

Additional HA Tag Products

Product Documents for HA Tag Antibody (RM305)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HA Tag Antibody (RM305)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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