HA Tag Antibody (RMH02) [Janelia Fluor® 669]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26050JF669
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Western Blot
Label
Janelia Fluor 669
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # RMH02
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HA Tag Antibody (RMH02) [Janelia Fluor® 669]
Immunogen
This HA Tag Antibody (RMH02) was developed against a peptide corresponding to HA Tag (Human influenza hemagglutinin amino acids 98-106). This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of NBP2-61477 with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody.
Specificity
This antibody reacts to recombinant proteins containing a HA Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Applications for HA Tag Antibody (RMH02) [Janelia Fluor® 669]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA Capture
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA Detection
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: HA Tag
References
1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076
2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778
3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093
4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73
5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107
Alternate Names
HA Epitope Tag
Additional HA Tag Products
Product Documents for HA Tag Antibody (RMH02) [Janelia Fluor® 669]
Product Specific Notices for HA Tag Antibody (RMH02) [Janelia Fluor® 669]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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