HA Tag Antibody (RMH02) [Janelia Fluor® 669]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26050JF669

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Western Blot

Label

Janelia Fluor 669

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # RMH02

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HA Tag Antibodies »

Product Specifications

Immunogen

This HA Tag Antibody (RMH02) was developed against a peptide corresponding to HA Tag (Human influenza hemagglutinin amino acids 98-106). This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of NBP2-61477 with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody.

Specificity

This antibody reacts to recombinant proteins containing a HA Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Applications for HA Tag Antibody (RMH02) [Janelia Fluor® 669]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA Capture

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA Detection

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HA Tag

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Alternate Names

HA Epitope Tag

Additional HA Tag Products

Product Documents for HA Tag Antibody (RMH02) [Janelia Fluor® 669]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HA Tag Antibody (RMH02) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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