HA Tag Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-70006
![Western Blot: HA Tag Antibody [NBP1-70006]](https://resources.bio-techne.com/images/products/HA-Epitope-Tag-Antibody-Western-Blot-NBP1-70006-img0004.jpg "Western Blot: HA Tag Antibody [NBP1-70006]")
Key Product Details
Species Reactivity
Epitope Tag
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HA Tag Antibody was purified from whole rabbit serum prepared by repeated immunizations with the epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH.
Specificity
This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for HA Tag Antibody
Western Blot: HA Tag Antibody [NBP1-70006]
Western Blot: HA Tag Antibody [NBP1-70006] - Detection of 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.![Immunohistochemistry-Paraffin: HA Tag Antibody [NBP1-70006]](https://resources.bio-techne.com/images/products/HA-Epitope-Tag-Antibody-Immunohistochemistry-Paraffin-NBP1-70006-img0001.jpg "Immunohistochemistry-Paraffin: HA Tag Antibody [NBP1-70006]")
Immunohistochemistry-Paraffin: HA Tag Antibody [NBP1-70006]
Immunohistochemistry-Paraffin: HA Tag Antibody [NBP1-70006] - Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 um were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.
HA Tag Antibody
Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 um were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4 C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.Applications for HA Tag Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
ELISA
1:10000-1:100000
Immunohistochemistry
1:500-1:2000
Immunoprecipitation
1:10 - 1:500
Western Blot
1:2000-1:10000
Application Notes
This product is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA, immunohistochemistry, and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: HA Tag
References
1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076
2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778
3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093
4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73
5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107
Alternate Names
HA Epitope Tag
Additional HA Tag Products
Product Documents for HA Tag Antibody
Product Specific Notices for HA Tag Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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