HA Tag Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21581

Novus Biologicals, part of Bio-Techne
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NBP2-21581
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Conjugate
Catalog #
HRP
NBP2-16773
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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HA Tag Antibodies »

Product Summary for HA Tag Antibody

Immunogen

The immunogen used to generate this antibody corresponds to HA Tag

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HA Tag Antibody

Western Blot: HA Tag Antibody [NBP2-21581]

Western Blot: HA Tag Antibody [NBP2-21581]

Western Blot: HA Tag Antibody [NBP2-21581] - Analysis in human HEK293T cells overexpressed with Ly6K-HA. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: HA Tag Antibody [NBP2-21581]

Immunocytochemistry/ Immunofluorescence: HA Tag Antibody [NBP2-21581]

Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NBP2-21581] - Analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using antibody at 1:500 dilution.
Flow Cytometry: HA Tag Antibody [NBP2-21581]

Flow Cytometry: HA Tag Antibody [NBP2-21581]

Flow Cytometry: HA Tag Antibody [NBP2-21581] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted by a verified customer review.
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Applications for HA Tag Antibody

Application
Recommended Usage

ELISA

1:1000 - 1:10000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunoprecipitation

1:100 - 1:500

Western Blot

1:1000 - 1:10000
Application Notes
This HA Tag antibody is validated for Flow cytometry and WB from verified customer reviews.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP2-21581 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HA Tag

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Alternate Names

HA Epitope Tag

Additional HA Tag Products

Product Documents for HA Tag Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HA Tag Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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