HACE1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55062
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to HACE1(HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1) The peptide sequence was selected from the middle region of HACE1.
Peptide sequence DVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRV The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for HACE1 Antibody
![Western Blot: HACE1 Antibody [NBP1-55062]](https://resources.bio-techne.com/images/products/HACE1-Antibody-Western-Blot-NBP1-55062-img0003.jpg "Western Blot: HACE1 Antibody [NBP1-55062]")
Western Blot: HACE1 Antibody [NBP1-55062]
Western Blot: HACE1 Antibody [NBP1-55062] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.![Western Blot: HACE1 Antibody [NBP1-55062]](https://resources.bio-techne.com/images/products/HACE1-Antibody-Western-Blot-NBP1-55062-img0004.jpg "Western Blot: HACE1 Antibody [NBP1-55062]")
Western Blot: HACE1 Antibody [NBP1-55062]
Western Blot: HACE1 Antibody [NBP1-55062] - Sample Tissue: Hela Whole cell, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/Lane, Gel Concentration: 0.12Applications for HACE1 Antibody
Application
Recommended Usage
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HACE1
Alternate Names
E3 ubiquitin-protein ligase HACE1, EC 6.3.2, HACE, HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1, HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1, KIAA1320EC 6.3.2.-
Gene Symbol
HACE1
UniProt
Additional HACE1 Products
Product Documents for HACE1 Antibody
Product Specific Notices for HACE1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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