HAND2 Antibody (3E3)

Novus Biologicals, part of Bio-Techne | Catalog # H00009464-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3E3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HAND2 Antibodies »

Product Specifications

Immunogen

HAND2 (NP_068808.1, 135 a.a. ~ 216 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LSKIKTLRLATSYIAYLMDLLAKDDQNGEAEAFKAEIKKTDVKEEKRKKELNEILKSTVSSNDKKTKGRTGWPQHVWALELK

Specificity

HAND2 (3E3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HAND2 Antibody (3E3)

Western Blot: HAND2 Antibody (3E3) [H00009464-M03]

Western Blot: HAND2 Antibody (3E3) [H00009464-M03]

Western Blot: HAND2 Antibody (3E3) [H00009464-M03] - Analysis of HAND2 expression in transfected 293T cell line by HAND2 monoclonal antibody (M03), clone 3E3. Lane 1: HAND2 transfected lysatE (21.6 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: HAND2 Antibody (3E3) [H00009464-M03]

Immunocytochemistry/ Immunofluorescence: HAND2 Antibody (3E3) [H00009464-M03]

Immunocytochemistry/Immunofluorescence: HAND2 Antibody (3E3) [H00009464-M03] - Analysis of monoclonal antibody to HAND2 on HeLa cell. Antibody concentration 10 ug/ml

Applications for HAND2 Antibody (3E3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HAND2

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.

Long Name

Heart And Neural Crest Derivatives Expressed 2

Alternate Names

dHand, Ehand2, Hed, Th2, Thing2

Entrez Gene IDs

9464 (Human)

Gene Symbol

HAND2

OMIM

602407 (Human)

UniProt

NP_068808 (Human)

Additional HAND2 Products

Product Documents for HAND2 Antibody (3E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HAND2 Antibody (3E3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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