HARS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16781

Novus Biologicals, part of Bio-Techne
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NBP2-16781
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HARS Antibodies »

Product Summary for HARS Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human HARS. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HARS Antibody

Western Blot: HARS Antibody [NBP2-16781]

Western Blot: HARS Antibody [NBP2-16781]

Western Blot: HARS Antibody [NBP2-16781] - A. 30 ug HepG2 whole cell lysate/extract B. 30 ug HCT116 whole cell lysate/extract 7. 5 % SDS-PAGE HARS antibody [N1C1] dilution: 1:500.
Immunohistochemistry-Paraffin: HARS Antibody [NBP2-16781]

Immunohistochemistry-Paraffin: HARS Antibody [NBP2-16781]

Immunohistochemistry-Paraffin: HARS Antibody [NBP2-16781] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Applications for HARS Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HARS

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq]

Alternate Names

cytoplasmic, EC 6.1.1.21, FLJ20491, histidine-tRNA ligase, histidyl-tRNA synthetase, histidyl-tRNA synthetase, cytoplasmic

Gene Symbol

HARS1

UniProt

P12081 (Human)

Additional HARS Products

Product Documents for HARS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HARS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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