HB-EGF Antibody (S08-9C2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15039

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15039-100ul
NBP3-15039-25ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-9C2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HB-EGF Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human HB-EGF (Uniprot#: Q99075)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HB-EGF Antibody (S08-9C2)

Western Blot: HB-EGF Antibody (S08-9C2) [NBP3-15039]

Western Blot: HB-EGF Antibody (S08-9C2) [NBP3-15039]

Western Blot: HB-EGF Antibody (S08-9C2) [NBP3-15039] - Western blot detection of HB-EGF in K562, C6, 3T3, Hela cell lysates using HB-EGF Rabbit mAb (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 23kDa.

Applications for HB-EGF Antibody (S08-9C2)

Application
Recommended Usage

Western Blot

1/2000-1/10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HB-EGF

HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein.

Long Name

Heparin Binding EGF-like Growth Factor

Alternate Names

Dtr, Dts, HBEGF, Hegfl

Gene Symbol

HBEGF

Additional HB-EGF Products

Product Documents for HB-EGF Antibody (S08-9C2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HB-EGF Antibody (S08-9C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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