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Hck Antibody (1D9-1A9)

Novus Biologicals, part of Bio-Techne | Catalog # H00003055-M01

Novus Biologicals, part of Bio-Techne
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H00003055-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D9-1A9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HCK (AAH14435, 1 a.a. ~ 505 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCMKSKFLQVGGNTSSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGVSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP

Localization

Isoform p60-HCK and isoform p59-HCK are associated with membranes. Isoform p60-HCK is also cytoplasmic.

Specificity

HCK - hemopoietic cell kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Hck Antibody (1D9-1A9)

Western Blot: Hck Antibody (1D9-1A9) [H00003055-M01]

Western Blot: Hck Antibody (1D9-1A9) [H00003055-M01]

Western Blot: Hck Antibody (1D9-1A9) [H00003055-M01] - Analysis of HCK expression in transfected 293T cell line by HCK monoclonal antibody (M01), clone 1D9-1A9.Lane 1: HCK transfected lysate (Predicted MW: 57.3 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: Hck Antibody (1D9-1A9) [H00003055-M01]

Immunoprecipitation: Hck Antibody (1D9-1A9) [H00003055-M01]

Immunoprecipitation: Hck Antibody (1D9-1A9) [H00003055-M01] - Analysis of HCK transfected lysate using anti-HCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HCK MaxPab rabbit polyclonal antibody.

Applications for Hck Antibody (1D9-1A9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hck

The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

Long Name

Hemopoietic Cell Kinase

Alternate Names

Bmk, Hctk, JTK9

Entrez Gene IDs

3055 (Human)

Gene Symbol

HCK

OMIM

142370 (Human)

Additional Hck Products

Product Documents for Hck Antibody (1D9-1A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hck Antibody (1D9-1A9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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