Hck Antibody (2A6)
Novus Biologicals, part of Bio-Techne | Catalog # H00003055-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2A6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HCK (AAH14435, 1 a.a. ~ 80 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCMKSKFLQVGGNTFSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQK
Localization
Isoform p60-HCK and isoform p59-HCK are associated with membranes. Isoform p60-HCK is also cytoplasmic.
Specificity
HCK - hemopoietic cell kinase
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Hck Antibody (2A6)
![Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02]](https://resources.bio-techne.com/images/products/Hck-Antibody-2A6-Proximity-Ligation-Assay-H00003055-M02-img0002.jpg "Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02]")
Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02]
Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02] - Analysis of protein-protein interactions between FLT1 and HCK. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-HCK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: Hck Antibody (2A6) [H00003055-M02] - Detection limit for recombinant GST tagged HCK is approximately 3ng/ml as a capture antibody.
Applications for Hck Antibody (2A6)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Hck
Long Name
Hemopoietic Cell Kinase
Alternate Names
Bmk, Hctk, JTK9
Entrez Gene IDs
3055 (Human)
Gene Symbol
HCK
OMIM
142370 (Human)
UniProt
Additional Hck Products
Product Documents for Hck Antibody (2A6)
Product Specific Notices for Hck Antibody (2A6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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