Hck Antibody (S02-5C1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14957

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14957-100ul
NBP3-14957-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-5C1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Hck Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Hck (Uniprot#: P08631)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hck Antibody (S02-5C1)

Western Blot: Hck Antibody (S02-5C1) [NBP3-14957]

Western Blot: Hck Antibody (S02-5C1) [NBP3-14957]

Western Blot: Hck Antibody (S02-5C1) [NBP3-14957] - Western blot detection of Hck in Hela, A549, HL-60, U2OS cell lysates using Hck Rabbit mAb (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.
Immunocytochemistry/ Immunofluorescence: Hck Antibody (S02-5C1) [NBP3-14957]

Immunocytochemistry/ Immunofluorescence: Hck Antibody (S02-5C1) [NBP3-14957]

Immunocytochemistry/Immunofluorescence: Hck Antibody (S02-5C1) [NBP3-14957] - Immunocytochemistry of Hck (green) in hela using Hck Rabbit mAb at dilution 1/50, and DAPI (blue)

Applications for Hck Antibody (S02-5C1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/2000-1/10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hck

The 60-kilodalton protein encoded by HCK resembles the product of the proto-oncogene c-src and is therefore likely to be a peripheral membrane protein (1). Protein-tyrosine kinase Hck is primarily expressed in hematopoietic cells, particularly granulocytes. Protein-tyrosine kinases are implicated in the control of cell growth by virtue of their frequent appearance as products of retroviral oncogenes and as components of growth factor receptors (2). Tyrosine kinases of the Src family are regulated via their Src homology 2 (SH2) and SH3 domains. The Nef protein of human immunodeficiency virus-1 (HIV-1) has previously been shown to bind with high affinity and specificity in vitro to the SH3 domain of Hck, a Src family member expressed primarily in myeloid cells. Results provide direct evidence that SH3 engagement is sufficient to activate a Src family kinase in vivo and suggest that Hck may be activated by Nef in HIV-infected macrophages (3).

Long Name

Hemopoietic Cell Kinase

Alternate Names

Bmk, Hctk, JTK9

Gene Symbol

HCK

Additional Hck Products

Product Documents for Hck Antibody (S02-5C1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hck Antibody (S02-5C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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