HCN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22518

Novus Biologicals, part of Bio-Techne
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NBP2-22518
NBP2-22518-0.025mg
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Key Product Details

Species Reactivity

Validated:

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all HCN2 Antibodies »

Product Summary for HCN2 Antibody

Immunogen

Synthetic Peptide: G(874) L D P Q D S A R S R L S S N L(889)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HCN2 Antibody

Western Blot: HCN2 Antibody [NBP2-22518]

Western Blot: HCN2 Antibody [NBP2-22518]

Western Blot: HCN2 Antibody [NBP2-22518] - Analysis of HCN2 in rat brain extracts.

Applications for HCN2 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
WB: Detects an approx. 100 kDa and 115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HCN2

Hyperpolarization-activated cation channels of the HCN gene family such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems. FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in neonatal and adult ventricle and in brain. Highly expressed in the pyramidal layer in hippocampus, in anterior dorsal nucleus in thalamus, in the mammillary nucleus in hypothalamus, in red nucleus, in trigeminal mesencephalic, spinal and principal nuclei, in cochlear and trapezoid nuclei and in the dorsal tegemental nucleus. INDUCTION: By thyroid hormones in hypothyroid animals. DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Long Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Alternate Names

BCNG-2, BCNG2, EIG17, GEFSP11, HAC-1

Entrez Gene IDs

114244 (Rat)

Gene Symbol

HCN2

Additional HCN2 Products

Product Documents for HCN2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HCN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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