HDAC1 Antibody (2L6O7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15780

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15780-100ul
NBP3-15780-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2L6O7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 1/HDAC1 Antibodies »

Product Summary for HDAC1 Antibody (2L6O7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human HDAC1 (Q13547). MTNQNTNEYLEKIKQRLFENLRMLPHAPGVQMQAIPEDAIPEESGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC1 Antibody (2L6O7)

Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780]

Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780]

Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (2L6O7) [NBP3-15780]

Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (2L6O7) [NBP3-15780]

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (2L6O7) [NBP3-15780] - Immunofluorescence analysis of NIH/3T3 cells using HDAC1 antibody (NBP3-15780) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: HDAC1 Antibody (2L6O7) [NBP3-15780]

Immunohistochemistry-Paraffin: HDAC1 Antibody (2L6O7) [NBP3-15780]

Immunohistochemistry-Paraffin: HDAC1 Antibody (2L6O7) [NBP3-15780] - Immunohistochemistry of paraffin-embedded Mouse brain using HDAC1 antibody (NBP3-15780) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for HDAC1 Antibody (2L6O7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 1/HDAC1

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Long Name

Histone Deacetylase 1

Alternate Names

GON-10, HD1, KDAC1, RPD3L1

Gene Symbol

HDAC1

Additional Histone Deacetylase 1/HDAC1 Products

Product Documents for HDAC1 Antibody (2L6O7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HDAC1 Antibody (2L6O7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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