HDAC11 Antibody

Knockdown Validated: HDAC11 Antibody [NBP2-16789]

Knockdown Validated: HDAC11 Antibody [NBP2-16789] - HDAC11 silencing decreases expression of downstream targets and abrogates self-renewal of CSCs. Depletion of HDAC11 using two IPTG inducible shRNA shows a significant decrease in Sox2, YAP1 and Gli1 protein expression as compared to IPTG treated control shRNA. The glycolysis pathway targets HK2 and PDK2 also show reduced protein expression in the absence of HDAC11. No change is observed in PDK1 expression; the full images of the western blots are provided as Supplementary Fig. 4. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-020-61295-6) licensed under a CC-BY license.

Western Blot: HDAC11 Antibody [NBP2-16789] -

Western Blot: HDAC11 Antibody [NBP2-16789] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC11 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HDAC11 Antibody [NBP2-16789] -

Western Blot: HDAC11 Antibody [NBP2-16789] - HDAC11 silencing decreases expression of downstream targets & abrogates self-renewal of CSCs. (A) Depletion of HDAC11 using two IPTG inducible shRNA shows a significant decrease in Sox2, YAP1 & Gli1 protein expression as compared to IPTG treated control shRNA. The glycolysis pathway targets HK2 & PDK2 also show reduced protein expression in the absence of HDAC11. No change is observed in PDK1 expression; the full images of the western blots are provided as Supplementary Fig. 4. (B) Quantitation of the western blot band intensities using ImageJ analysis. (C) Sphere formation assay with SP cells from shHDAC11 H1650 cells treated with IPTG show abrogation of self-renewal ability in the absence of HDAC11. (D) Quantitation of sphere formation assay reveal that IPTG treated SP cells from two different clones of HDAC11 shRNA containing H1650 form fewer spheres as compared to IPTG untreated or control shRNA containing cells. (E,F) Real time PCR analysis of H1650 cells with two different HDAC11 shRNA show a decrease in mRNA expression of Sox2, HK2, PDK1, PDK2 with IPTG treatment. No significant change was observed in Oct4 & Nanog expression. The depletion of HDAC11 was confirmed using RT-PCR. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32170113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.