HDAC3 Antibody (1M1O7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15760

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15760-100ul
NBP3-15760-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1M1O7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 3/HDAC3 Antibodies »

Product Summary for HDAC3 Antibody (1M1O7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 329-428 of human HDAC3 (NP_003874.2). FEYFAPDFTLHPDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADLLTYDRTDEADAEERGPEENYSRPEAPNEFYDGDHDNDKESDVEI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC3 Antibody (1M1O7)

Western Blot: HDAC3 Antibody (1M1O7) [NBP3-15760]

Western Blot: HDAC3 Antibody (1M1O7) [NBP3-15760]

Western Blot: HDAC3 Antibody (1M1O7) [NBP3-15760] - Western blot analysis of extracts of various cell lines, using HDAC3 antibody (NBP3-15760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
Immunoprecipitation: HDAC3 Antibody (1M1O7) [NBP3-15760]

Immunoprecipitation: HDAC3 Antibody (1M1O7) [NBP3-15760]

Immunoprecipitation: HDAC3 Antibody (1M1O7) [NBP3-15760] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC3 antibody (NBP3-15760). Western blot was performed from the immunoprecipitate using HDAC3 (NBP3-15760) at a dilition of 1:1000.

Applications for HDAC3 Antibody (1M1O7)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 3/HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Long Name

Histone Deacetylase 3

Alternate Names

HDAC3, KDAC3, RPD3-2, SMAP45

Gene Symbol

HDAC3

Additional Histone Deacetylase 3/HDAC3 Products

Product Documents for HDAC3 Antibody (1M1O7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HDAC3 Antibody (1M1O7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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