HDAC3 Antibody (4D4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26237
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4D4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HDAC3 Antibody (4D4)
Immunogen
A synthesized peptide derived from Human HDAC3 [UniProt O15379]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HDAC3 Antibody (4D4)
Immunohistochemistry: HDAC3 Antibody (4D4) [NBP3-26237] -
Immunohistochemistry: HDAC3 Antibody (4D4) [NBP3-26237] - Image of HDAC3 Antibody (4D4) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: HDAC3 Antibody (4D4) [NBP3-26237] -
Immunohistochemistry: HDAC3 Antibody (4D4) [NBP3-26237] - Image of HDAC3 Antibody (4D4) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: HDAC3 Antibody (4D4) [NBP3-26237] -
Western Blot: HDAC3 Antibody (4D4) [NBP3-26237] - Positive Western Blot detected in: Hela whole cell lysate, HL60 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate, 293T whole cell lysate.All lanes: HDAC3 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa
Applications for HDAC3 Antibody (4D4)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 3/HDAC3
Long Name
Histone Deacetylase 3
Alternate Names
HDAC3, KDAC3, RPD3-2, SMAP45
Gene Symbol
HDAC3
Additional Histone Deacetylase 3/HDAC3 Products
Product Documents for HDAC3 Antibody (4D4)
Product Specific Notices for HDAC3 Antibody (4D4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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