HDAC3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31196
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Drosophila
Predicted:
Bovine (100%), Chicken (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HDAC3. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HDAC3 Antibody
Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody [NBP1-31196]
Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NBP1-31196] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term diluted at 1:400. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196]
Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196] - SW480 xenograft, using HDAC3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Chromatin Immunoprecipitation: HDAC3 Antibody [NBP1-31196] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC3 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
Applications for HDAC3 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 3/HDAC3
Long Name
Histone Deacetylase 3
Alternate Names
HDAC3, KDAC3, RPD3-2, SMAP45
Gene Symbol
HDAC3
Additional Histone Deacetylase 3/HDAC3 Products
Product Documents for HDAC3 Antibody
Product Specific Notices for HDAC3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.