HDAC5 Antibody (CL8121) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44217

Novus Biologicals, part of Bio-Techne
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NBP3-44217
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Catalog #
BSA Free
NBP2-88913
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL8121

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Histone Deacetylase 5/HDAC5 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q9UQL6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HDAC5 Antibody (CL8121) - Azide and BSA Free

HDAC5 Antibody (CL8121) - Azide and BSA Free Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP3-44217]

Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP3-44217]

Analysis in human cerebral cortex and liver tissues using NBP3-44217 antibody. Corresponding HDAC5 RNA-seq data are presented for the same tissues.
HDAC5 Antibody (CL8121) - Azide and BSA Free Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP3-44217]

Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP3-44217]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
HDAC5 Antibody (CL8121) - Azide and BSA Free Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP3-44217]

Immunohistochemistry-Paraffin: HDAC5 Antibody [NBP3-44217]

Staining of human liver shows no positivity in hepatocytes as expected.
View 4 more images

Applications for HDAC5 Antibody (CL8121) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 5/HDAC5

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF(p300/CBP- associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Long Name

Histone Deacetylase 5

Alternate Names

FLJ90614, KIAA0600, NY-CO-9

Gene Symbol

HDAC5

Additional Histone Deacetylase 5/HDAC5 Products

Product Documents for HDAC5 Antibody (CL8121) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HDAC5 Antibody (CL8121) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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