HDAC6 Antibody (9V1W2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16082
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9V1W2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HDAC6 Antibody (9V1W2)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC6 (Q9UBN7). MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HDAC6 Antibody (9V1W2)
![Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082]](https://resources.bio-techne.com/images/products/HDAC6-Antibody-9V1W2-Western-Blot-NBP3-16082-img0002.jpg "Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082]")
Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082]
Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082] - Western blot analysis of extracts of various cell lines, using HDAC6 antibody (NBP3-16082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082]](https://resources.bio-techne.com/images/products/HDAC6-Antibody-9V1W2-Immunohistochemistry-Paraffin-NBP3-16082-img0001.jpg "Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082]")
Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082]
Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082] - Immunohistochemistry of paraffin-embedded human breast cancer using HDAC6 Rabbit mAb (NBP3-16082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082]](https://resources.bio-techne.com/images/products/HDAC6-Antibody-9V1W2-Immunoprecipitation-NBP3-16082-img0003.jpg "Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082]")
Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082]
Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC6 antibody (NBP3-16082). Western blot was performed from the immunoprecipitate using HDAC6 antibody (NBP3-16082) at a dilition of 1:1000.Applications for HDAC6 Antibody (9V1W2)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 6/HDAC6
Long Name
Histone Deacetylase 6
Alternate Names
CPBHM, FLJ16239, HD6, HDAC6, JM21, PPP1R90
Gene Symbol
HDAC6
Additional Histone Deacetylase 6/HDAC6 Products
Product Documents for HDAC6 Antibody (9V1W2)
Product Specific Notices for HDAC6 Antibody (9V1W2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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