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HDAC7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98759

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human HDAC7.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for HDAC7 Antibody

Western Blot: HDAC7 Antibody [NBP2-98759]

Western Blot: HDAC7 Antibody [NBP2-98759]

Western Blot: HDAC7 Antibody [NBP2-98759] - Anti-HDAC7 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa
Immunoprecipitation: HDAC7 Antibody [NBP2-98759]

Immunoprecipitation: HDAC7 Antibody [NBP2-98759]

Immunoprecipitation: HDAC7 Antibody [NBP2-98759] - HDAC7 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 1 ul anti-HDAC7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HDAC7 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 103 kDa. Observed band size: 103 kDa

Applications for HDAC7 Antibody

Application
Recommended Usage

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDAC7

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Long Name

Histone deacetylase 7

Alternate Names

HD7, HD7a, HDAC7A

Gene Symbol

HDAC7

Additional HDAC7 Products

Product Documents for HDAC7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HDAC7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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