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HDGFRP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13246

Novus Biologicals, part of Bio-Techne
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NBP2-13246
NBP2-13246-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: LWEIENNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDGFRP3 Antibody

Western Blot: HDGFRP3 Antibody [NBP2-13246]

Western Blot: HDGFRP3 Antibody [NBP2-13246]

Western Blot: HDGFRP3 Antibody [NBP2-13246] - Analysis in control (vector only transfected HEK293T lysate) and HDGFRP3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: HDGFRP3 Antibody [NBP2-13246]

Immunocytochemistry/ Immunofluorescence: HDGFRP3 Antibody [NBP2-13246]

Immunocytochemistry/Immunofluorescence: HDGFRP3 Antibody [NBP2-13246] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HDGFRP3 Antibody [NBP2-13246]

Immunohistochemistry-Paraffin: HDGFRP3 Antibody [NBP2-13246]

Immunohistochemistry-Paraffin: HDGFRP3 Antibody [NBP2-13246] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.

Applications for HDGFRP3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF,Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HDGFRP3

Enhances DNA synthesis and may play a role in cell proliferation

Alternate Names

HDGF2, HDGF-2, Hepatoma-derived growth factor 2, hepatoma-derived growth factor, related protein 3, hepatoma-derived growth factor-related protein 3, HRP-3

Gene Symbol

HDGFRP3

Additional HDGFRP3 Products

Product Documents for HDGFRP3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HDGFRP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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