HE4/WFDC2 Antibody (17D2)

Western Blot: HE4/WFDC2 Antibody (17D2) [NBP3-26252] -

Western Blot: HE4/WFDC2 Antibody (17D2) [NBP3-26252] - Positive Western Blot detected in: HT29 whole cell lysate, Hela whole cell lysate, HT29 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate.
All lanes: HE4/WFDC2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 25 kDa
Observed band size: 25 kDa

Flow Cytometry: HE4/WFDC2 Antibody (17D2) [NBP3-26252] -

Flow Cytometry: HE4/WFDC2 Antibody (17D2) [NBP3-26252] - Overlay Peak curve showing MCF-7 cells stained with HE4/WFDC2 Antibody (17D2) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.