HE4/WFDC2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27299

Novus Biologicals, part of Bio-Techne
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NBP2-27299
NBP2-27299SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all HE4/WFDC2 Antibodies »

Product Summary for HE4/WFDC2 Antibody

Immunogen

A portion of amino acids 1-50 of (human) HE4 was used as the immunogen for the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HE4/WFDC2 Antibody

Western Blot: HE4/WFDC2 Antibody [NBP2-27299]

Western Blot: HE4/WFDC2 Antibody [NBP2-27299]

Western Blot: HE4/WFDC2 Antibody [NBP2-27299] - Ovarian tumor line SKOV3 probed with HE4 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP2-27299]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP2-27299]

Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody [NBP2-27299] - Formalin-fixed, paraffin-embedded normal breast tissue stained with HE4 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note ductal staining.

Applications for HE4/WFDC2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-7ug/ml~

Western Blot

1-3ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HE4/WFDC2

HE4, or human epididymis protein 4, is overexpressed in ovarian carcinomas (1-3). Ovarian cancer represents one disease where cure rate can be increased if the disease is diagnosed early. While the most routinely used serum marker is CA-125, this marker is elevated in only about 50% in patients in early disease stages. Conversely, by ELISA, HE4 detectable levels in serum appear far more frequently in patients with nonmalignant or early disease stages unlike CA-125. So whereas HE4 levels are elevated in about 90% of woman with ovarian cancer, HE4 is not frequently overexpressed as is CA-125 in patients with benign gynecologic diseases (4). Additionally, in conjunction with CA-125 and another serum marker, mesothelin, HE4 adds to a promising combination for earlier detection and diagnosis of ovarian cancer. Antibody to HE4 provides a useful tool for detection and potential quantization and further assessment of more in depth potential of HE4 as a biomarker in ovarian cancer.

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Gene Symbol

WFDC2

UniProt

Q14508 (Human)

Additional HE4/WFDC2 Products

Product Documents for HE4/WFDC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HE4/WFDC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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