HECTD1 Antibody (1E10)
Novus Biologicals, part of Bio-Techne | Catalog # H00025831-M03
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1E10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HECTD1 (NP_056197, 3 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DVDPDTLLEWLQMGQGDERDMQLIALEQLCMLLLMSDNVDRCFETCPPRTFLPALCKIFLDESAPDNVLEVTARAITYYLDVSAECTRRIVGVDGAIKALCNRLVVVE
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24855001) Other species not tested. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
HECTD1 - HECT domain containing 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for HECTD1 Antibody (1E10)
![ELISA: HECTD1 Antibody (1E10) [H00025831-M03]](https://resources.bio-techne.com/images/products/HECTD1-Antibody-1E10-ELISA-H00025831-M03-img0001.jpg "ELISA: HECTD1 Antibody (1E10) [H00025831-M03]")
ELISA: HECTD1 Antibody (1E10) [H00025831-M03]
ELISA: HECTD1 Antibody (1E10) [H00025831-M03] - Detection limit for recombinant GST tagged HECTD1 is approximately 0.3ng/ml as a capture antibody.Applications for HECTD1 Antibody (1E10)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24855001). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 22431752)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HECTD1
Alternate Names
E3 ligase for inhibin receptor, E3 ubiquitin-protein ligase HECTD1, EC 6.3.2.-, EULIR, HECT domain containing 1, HECT domain-containing protein 1, KIAA1131FLJ38315
Entrez Gene IDs
25831 (Human)
Gene Symbol
HECTD1
UniProt
Additional HECTD1 Products
Product Documents for HECTD1 Antibody (1E10)
Product Specific Notices for HECTD1 Antibody (1E10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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