HECTD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83481

Novus Biologicals, part of Bio-Techne
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NBP1-83481
NBP1-83481-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HECTD2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPAHLVFPNIKNVREPPPICLDVRQKQRTSMDASSSEMKAPVLPEPILPIQPKTVKDFQEDVEKVKSSGDWKA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HECTD2 Antibody

Western Blot: HECTD2 Antibody [NBP1-83481]

Western Blot: HECTD2 Antibody [NBP1-83481]

Western Blot: HECTD2 Antibody [NBP1-83481] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: HECTD2 Antibody [NBP1-83481]

Immunocytochemistry/ Immunofluorescence: HECTD2 Antibody [NBP1-83481]

Immunocytochemistry/Immunofluorescence: HECTD2 Antibody [NBP1-83481] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HECTD2 Antibody [NBP1-83481]

Immunohistochemistry-Paraffin: HECTD2 Antibody [NBP1-83481]

Immunohistochemistry-Paraffin: HECTD2 Antibody [NBP1-83481] - Staining of human Rectum shows strong cytoplasmic positivity in glandular cells.
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Applications for HECTD2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HECTD2

Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in theform of a thioester and then directly transfers the ubiquitin to targeted substrates

Long Name

HECT Domain E3 Ubiquitin Protein Ligase 2

Alternate Names

EC2.3.2.26

Gene Symbol

HECTD2

Additional HECTD2 Products

Product Documents for HECTD2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HECTD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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