Hemogen Antibody (OTI2D7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72005

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Hemogen Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 260-484 of human HEMGN (NP_060907) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hemogen Antibody (OTI2D7) - Azide and BSA Free

Western Blot: Hemogen Antibody (OTI2D7)Azide and BSA Free [NBP2-72005]

Western Blot: Hemogen Antibody (OTI2D7)Azide and BSA Free [NBP2-72005]

Western Blot: Hemogen Antibody (OTI2D7) - Azide and BSA Free [NBP2-72005] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hemogen.
Immunohistochemistry: Hemogen Antibody (OTI2D7) - Azide and BSA Free [NBP2-72005]

Immunohistochemistry: Hemogen Antibody (OTI2D7) - Azide and BSA Free [NBP2-72005]

Immunohistochemistry: Hemogen Antibody (OTI2D7) - Azide and BSA Free [NBP2-72005] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Hemogen Antibody (OTI2D7) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hemogen

Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB)

Alternate Names

EDAGEDAG-1, Erythroid differentiation-associated gene protein, hemogen, Hemopoietic gene protein, NDR, Negative differentiation regulator protein

Gene Symbol

HEMGN

Additional Hemogen Products

Product Documents for Hemogen Antibody (OTI2D7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Hemogen Antibody (OTI2D7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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