Hemoglobin alpha Antibody (PD00-16)

Western Blot: Hemoglobin alpha Antibody (PD00-16) [NBP3-32421]

Western Blot: Hemoglobin alpha Antibody (PD00-16) [NBP3-32421] - Western blot analysis of Hemoglobin alpha on rat heart tissue lysates with Rabbit anti-Hemoglobin alpha antibody (NBP3-32421) at 1/2,000 dilution.

Lysates/proteins at 20 ug/Lane.

Predicted band size: 15 kDa
Observed band size: 12 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32421) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Hemoglobin alpha Antibody (PD00-16) [NBP3-32421]

Immunohistochemistry: Hemoglobin alpha Antibody (PD00-16) [NBP3-32421] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Rabbit anti-Hemoglobin alpha antibody (NBP3-32421) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody () at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.