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Hemoglobin Antibody (2A1) [Alexa Fluor® 532] - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20120AF532

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Alexa Fluor 532

Antibody Source

Monoclonal Rabbit IgG Kappa Clone # 2A1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend's Adjuvant.

Specificity

2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 from the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for Hemoglobin Antibody (2A1) [Alexa Fluor® 532] - Chimeric

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Hemoglobin

Hemoglobin is a tetrameric protein that serves as the oxygen transporter in red blood cells. Two alpha chain and two beta chains comprise the adult form of the molecule, with each subunit containing a heme group, or oxygen binding site. Sickle cell anemia is caused by a single amino acid change in the beta chain, which alters the solubility of the deoxygenated form of the molecule and causes the formation of long fibers that distort the red blood cell into the characteristic sickle shape. Thalassemias, another set of genetic diseases associated with hemoglobin, result from absence or deficiency in one of the hemoglobin chains.

Alternate Names

alpha one globin;alpha-1 globin;alpha-1-globin;alpha-2 globin chain;Alpha-globin;ALPHA-GLOBIN GENE;delta globin;Haemoglobin;Hb;HbA1c;hemoglobin alpha 1 globin chain;Hemoglobin alpha chain;Hemoglobin subunit alpha;hemoglobin, alpha 1

Additional Hemoglobin Products

Product Documents for Hemoglobin Antibody (2A1) [Alexa Fluor® 532] - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hemoglobin Antibody (2A1) [Alexa Fluor® 532] - Chimeric



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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