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Heparan Sulfate Proteoglycan 2 Antibody (A71) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23522

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Bovine

Applications

ELISA, Immunoaffinity Purification, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A71

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Bovine corneal endothelial cells

Epitope

Epitope is located in domain I

Specificity

Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Heparan Sulfate Proteoglycan 2 Antibody (A71) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
ELISA: NBP2-23522 can be used in ELISA. WB: In Western blotting a dilution guideline of 1/100 has proved successful (1,2) IHC: NBP2-23522 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP2-23522 can be used in affinity chromatography to separate recombinant domain I from tissue culture fluid. (1) IP: NBP2-23522 can be used in immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Heparan Sulfate Proteoglycan 2

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling .

Alternate Names

basement membrane-specific heparan sulfate proteoglycan core protein, endorepellin (domain V region), heparan sulfate proteoglycan 2, HSPG, perlecan, perlecan proteoglycan, PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia), PRCAN, SJA, SJS, SJS1

Gene Symbol

HSPG2

Additional Heparan Sulfate Proteoglycan 2 Products

Product Documents for Heparan Sulfate Proteoglycan 2 Antibody (A71) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Heparan Sulfate Proteoglycan 2 Antibody (A71) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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