Heparanase/HPSE Antibody (SR2148)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21871

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21871-100ul
NBP3-21871-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2148

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Heparanase/HPSE Antibodies »

Product Summary for Heparanase/HPSE Antibody (SR2148)

Immunogen

A synthesized peptide derived from human Heparanase/HPSE (Uniprot #: Q9Y251)

Specificity

Detects endogenous levels of total Heparanase/HPSE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Heparanase/HPSE Antibody (SR2148)

Western Blot: Heparanase/HPSE Antibody (SR2148) [NBP3-21871] -

Western Blot: Heparanase/HPSE Antibody (SR2148) [NBP3-21871] - Western blot analysis using NBP3-21871 in K562 cell lysate.

Applications for Heparanase/HPSE Antibody (SR2148)

Application
Recommended Usage

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Heparanase/HPSE

Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasations of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and an 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.

Alternate Names

Endo-glucoronidase, HPA1, HPR1, HPSE, HSE1

Gene Symbol

HPSE

Additional Heparanase/HPSE Products

Product Documents for Heparanase/HPSE Antibody (SR2148)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Heparanase/HPSE Antibody (SR2148)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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