Hepcidin Antimicrobial Peptide Antibody (1F9)

Novus Biologicals, part of Bio-Techne | Catalog # H00057817-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Hepcidin Antibodies »

Product Specifications

Immunogen

HAMP (AAH20612, 25 a.a. ~ 84 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SVFPQQTGQLAELQPQDRAGARASWMPMFQRRRRRDTHFPICIFCCGCCHRSKCGMCCKT

Specificity

HAMP - hepcidin antimicrobial peptide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Hepcidin Antimicrobial Peptide Antibody (1F9)

Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody (1F9) [H00057817-M02]

Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody (1F9) [H00057817-M02]

Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody (1F9) [H00057817-M02] - Immunoperoxidase of monoclonal antibody to HAMP ( Cat # H00057817-M02 ) on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
ELISA: Hepcidin Antimicrobial Peptide Antibody (1F9) [H00057817-M02]

ELISA: Hepcidin Antimicrobial Peptide Antibody (1F9) [H00057817-M02]

ELISA: Hepcidin Antimicrobial Peptide Antibody (1F9) [H00057817-M02] - Detection limit for recombinant GST tagged HAMP is approximately 0.03ng/ml as a capture antibody.

Applications for Hepcidin Antimicrobial Peptide Antibody (1F9)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hepcidin

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.

Long Name

Hepcidin Antimicrobial Peptide

Alternate Names

HAMP, HEPC, HFE2B, LEAP1, PLTR

Entrez Gene IDs

57817 (Human)

Gene Symbol

HAMP

OMIM

602390 (Human)

UniProt

AAH20612 (Human)

Additional Hepcidin Products

Product Documents for Hepcidin Antimicrobial Peptide Antibody (1F9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hepcidin Antimicrobial Peptide Antibody (1F9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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