HEXDC Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83574
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ASAFKGATGPSQAVPPVEHHLRNHVQWLQVAGSGPTDSLQGIILTGWQRYDHYSVLCELLPAGVPSLAACLQLLLRGGFDEDVKAKVEN
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HEXDC Antibody
Western Blot: HEXDC Antibody [NBP1-83574]
Western Blot: HEXDC Antibody [NBP1-83574] - Analysis in control (vector only transfected HEK293T lysate) and HEXDC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: HEXDC Antibody [NBP1-83574]
Immunocytochemistry/Immunofluorescence: HEXDC Antibody [NBP1-83574] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & mitochondria.Immunohistochemistry-Paraffin: HEXDC Antibody [NBP1-83574]
Immunohistochemistry-Paraffin: HEXDC Antibody [NBP1-83574] - Staining of human cerebellum shows strong cytoplasmic positivity in granular layer cells.Applications for HEXDC Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HEXDC
Alternate Names
Beta-hexosaminidase D, Beta-N-acetylhexosaminidase, EC 3.2.1.52, FLJ23825, hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing, hexosaminidase D, Hexosaminidase domain-containing protein, N-acetyl-beta-galactosaminidase
Gene Symbol
HEXDC
Additional HEXDC Products
Product Documents for HEXDC Antibody
Product Specific Notices for HEXDC Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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