Hexokinase 1 Antibody (3A10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51644

Novus Biologicals, part of Bio-Techne
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NBP1-51644
NBP1-51644-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A10

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Hexokinase 1 Antibodies »

Product Summary for Hexokinase 1 Antibody (3A10) - BSA Free

Immunogen

Purified recombinant fragment of human Hexokinase 1 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hexokinase 1 Antibody (3A10) - BSA Free

Western Blot: Hexokinase 1 Antibody (3A10)BSA Free [NBP1-51644]

Western Blot: Hexokinase 1 Antibody (3A10)BSA Free [NBP1-51644]

Western Blot: Hexokinase 1 Antibody (3A10) [NBP1-51644] - Analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (3A10) - BSA Free [NBP1-51644]

Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (3A10) - BSA Free [NBP1-51644]

Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (3A10) [NBP1-51644] - Analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (3A10) - BSA Free [NBP1-51644]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (3A10) - BSA Free [NBP1-51644]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (3A10) [NBP1-51644] - Analysis of kidney tissues using HK1 mouse mAb with DAB staining.
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Applications for Hexokinase 1 Antibody (3A10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexokinase 1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq]

Alternate Names

HK1, HKI

Entrez Gene IDs

3098 (Human)

Gene Symbol

HK1

UniProt

P19367

Additional Hexokinase 1 Products

Product Documents for Hexokinase 1 Antibody (3A10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hexokinase 1 Antibody (3A10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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