Hexokinase 1 Antibody (4D7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04295

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4D7

Format

BSA Free

Concentration

1.0 mg/ml

View all Hexokinase 1 Antibodies »

Product Summary for Hexokinase 1 Antibody (4D7) - BSA Free

Immunogen

Recombinant human Hexokinase1 (1-917aa) purified from E. coli

Specificity

The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Hexokinase 1 Antibody (4D7) - BSA Free

Western Blot: Hexokinase 1 Antibody (4D7)BSA Free [NBP1-04295]

Western Blot: Hexokinase 1 Antibody (4D7)BSA Free [NBP1-04295]

Western Blot: Hexokinase 1 Antibody (4D7) [NBP1-04295] - The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Hexokinase 1 recombinant protein Lane 2.: Hexokinase 2 recombinant protein Lane 3.: Hexokinase 3 recombinant protein Lane 4.: Hexokinase 4 recombinant protein
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (4D7) - BSA Free [NBP1-04295]

Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (4D7) - BSA Free [NBP1-04295]

Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (4D7) [NBP1-04295] - Human HeLa cells stained with Hoechst 33342 (Blue) and monoclonal antiHexokinase antibody (1:1000) with Alexa 488 (Green).
Western Blot: Hexokinase 1 Antibody (4D7)BSA Free [NBP1-04295]

Western Blot: Hexokinase 1 Antibody (4D7)BSA Free [NBP1-04295]

Western Blot: Hexokinase 1 Antibody (4D7) [NBP1-04295] - The tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Brain tissue lysate
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Applications for Hexokinase 1 Antibody (4D7) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexokinase 1

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for theparticular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.

Alternate Names

HK1, HKI

Entrez Gene IDs

3098 (Human)

Gene Symbol

HK1

UniProt

P19367

Additional Hexokinase 1 Products

Product Documents for Hexokinase 1 Antibody (4D7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Hexokinase 1 Antibody (4D7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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