Hexokinase 1 Antibody (6B7Q2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15274

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15274-100ul
NBP3-15274-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6B7Q2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Hexokinase 1 Antibodies »

Product Summary for Hexokinase 1 Antibody (6B7Q2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hexokinase 1 (P19367). GLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hexokinase 1 Antibody (6B7Q2)

Western Blot: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]

Western Blot: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]

Western Blot: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Western blot analysis of extracts of various cell lines, using Hexokinase 1 Rabbit mAb (NBP3-15274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]

Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]

Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunofluorescence analysis of U-2 OS cells using Hexokinase 1 Rabbit mAb (NBP3-15274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]

Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunohistochemistry of paraffin-embedded mouse kidney using Hexokinase 1 Rabbit mAb (NBP3-15274) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Hexokinase 1 Antibody (6B7Q2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hexokinase 1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Alternate Names

HK1, HKI

Gene Symbol

HK1

Additional Hexokinase 1 Products

Product Documents for Hexokinase 1 Antibody (6B7Q2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Hexokinase 1 Antibody (6B7Q2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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