Hexokinase 1 Antibody (6B7Q2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15274
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6B7Q2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hexokinase 1 (P19367). GLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Hexokinase 1 Antibody (6B7Q2)
Western Blot: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]
Western Blot: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Western blot analysis of extracts of various cell lines, using Hexokinase 1 Rabbit mAb (NBP3-15274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]
Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunofluorescence analysis of U-2 OS cells using Hexokinase 1 Rabbit mAb (NBP3-15274) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274]
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (6B7Q2) [NBP3-15274] - Immunohistochemistry of paraffin-embedded mouse kidney using Hexokinase 1 Rabbit mAb (NBP3-15274) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Hexokinase 1 Antibody (6B7Q2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Hexokinase 1
Alternate Names
HK1, HKI
Gene Symbol
HK1
Additional Hexokinase 1 Products
Product Documents for Hexokinase 1 Antibody (6B7Q2)
Product Specific Notices for Hexokinase 1 Antibody (6B7Q2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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