Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A7

Format

BSA Free

Concentration

1.0 mg/ml

View all Hexokinase 2 Antibodies »

Product Specifications

Immunogen

Recombinant human Hexokinase 2 (1-917aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Hexokinase 2 Antibody (1A7) - BSA Free

Western Blot: Hexokinase 2 Antibody (1A7)BSA Free [NBP1-04296]

Western Blot: Hexokinase 2 Antibody (1A7)BSA Free [NBP1-04296]

Western Blot: Hexokinase 2 Antibody (1A7) [NBP1-04296] - A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection.

Applications for Hexokinase 2 Antibody (1A7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexokinase 2

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.

Alternate Names

HK2, HKII, HXK2

Gene Symbol

HK2

UniProt

NP_000180 (Human)

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody (1A7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hexokinase 2 Antibody (1A7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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